Journal: PLoS ONE

Article Title: Low Cost Tuberculosis Vaccine Antigens in Capsules: Expression in Chloroplasts, Bio-Encapsulation, Stability and Functional Evaluation In Vitro

doi: 10.1371/journal.pone.0054708

Figure Lengend Snippet: Ls, Lactuca sativa ; Nt, Nicotiana tabacum ; pLD- vector components: the homologous Nicotiana tabacum chloroplast genome flanking sequence comprising of 16S 3′ end sequences and complete trn I, trn A genes; pLs- vector components: 16S trn I, the homologous Lactuca sativa chloroplast genome long flanking sequence containing 16S 3′end sequences and full length trn I gene; trn A 23S, trn A gene and 5′end of the 23S ribosomal RNA subunit; Prrn, ribosomal RNA operon promoter with GGAGG ribosome binding site; aad A, aminoglycoside 3′-adenylytransferase gene; TrbcL, 3′ untranslated region (Utr) of rbc L gene; PpsbA, promoter and 5′ Utr of psb A gene; TpsbA, 3′ Utr of psb A gene; CTB-ESAT6, coding sequence of cholera toxin B subunit fused to ESAT6 (6 kDa early secretory antigenic target); CTB-Mtb72F, coding sequence of cholera toxin B subunit fused to a recombinant fusion polyprotein from two TB antigens Mtb32 and Mtb39; LipY, coding sequence of a TB cell wall protein with lipase activity.

Article Snippet: Rabbit anti-cholera toxin B subunit polyclonal antibody (Abcam, ab34992) was coated on to washed protein A beads in a ratio of 50 µg/200 µl in 1X PBS overnight at 4°C for antibody binding to beads.

Techniques: Plasmid Preparation, Sequencing, Binding Assay, Recombinant, Activity Assay